
A Spectrophotometer is used to quantify levels of elements in compounds by measuring the amount of radiation absorbed at a specific wavelength
Welcome to the first of our blog posts explaining the function of various pieces of equipment you might find in a laboratory. A Spectrophotometer is used to quantify levels of elements in compounds by measuring the amount of radiation absorbed at a specific wavelength. There are several types of Spectrophotometer which are classified by the wavelength of radiation that they work at:-
- An IR Spectrophotometer works in the Infra-red region of around 10-2 to 10 -4 cm wavelength
- A UV-Vis Spectrophotometer works within the Ultra Violet to Visible region of around 10-4 to 10 -5cm wavelength
How do they work?
Basically, they consist of a source of light, a means to focus the beam of light, a sample container and some form of detector.
The light is emitted from the source at its given wavelength, then passes through a Monochromator to focus the beam so that only the waves that are travelling in a straight line from the source are allowed through the focus slit.
This wave then passes through the sample, where some of the light is absorbed and the detector then measures the difference in the light that has passed through the sample compared to the initial wave.

The light is emitted from the source at its given wavelength, then passes through a Monochromator
Are they only used in labs?
No, Spectrophotometers can be used in any situation where a change of colour could indicate a problem. However, they can only be used where the liquid involved is transparent. For example, they can be used in a bottling line for wine, beer or spirits. Here, the liquid passes through a Spectrophotometer to ensure that it blended/brewed/mixed consistently by falling within a colour specification.
They can also be used in the manufacture of tinted lenses for sunglasses to ensure that the colour of the lens is the same as the previous batch.
Have they changed over the years?
They have changed over the years as technology has advanced. As with many electrical items, developments in microchip technology have resulted in smaller Spectrophotometers.
They have also improved in terms of their flexibility. Whereas once you had to change a bulb to change the wavelength you were looking at, the newer models are far more flexible and can be controlled by the simple press of a button.
Do you have to be qualified to use one?
As basic understanding of what you are trying to achieve and what you are handling should be more than sufficient for anyone to operate a Spectrophotometer.
With a clearly defined set of instructions to follow, anyone could actually analyse a basic sample on a Spectrophotometer. However, qualifications in chemistry and physics would be advantageous when it comes to developing new methods and interpreting results.
How much do they cost?
Generally speaking, Spectrophotometers cost upwards of £5,000 and can cost tens of thousands. However, prices generally are decreasing due to the influx of more keenly priced electronics from Asia.
We use Spectrophotometers at ReAgent, the UK’s leading chemical suppliers.